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An oxygen-, acid- and anaesthetic-sensitive TASK-like background potassium channel in rat arterial chemoreceptor cells

Keith J. Buckler, Beatrice A. Williams and Eric Honore*

1. The biophysical and pharmacological properties of an oxygen-sensitive background K⁺ current in rat carotid body type-I cells were investigated and compared with those of recently cloned two pore domain K⁺ channels.

2. Under symmetrical K⁺ conditions the oxygen-sensitive whole cell K⁺ current had a linear dependence on voltage indicating a lack of intrinsic voltage sensitivity.

3. Single channel recordings identified a K⁺ channel, open at resting membrane potentials, that was inhibited by hypoxia. This channel had a single channel conductance of 14 pS, flickery kinetics and showed little voltage sensitivity except at extreme positive potentials.

4. Oxygen-sensitive current was inhibited by 10 mM barium (57 % inhibition), 200 µM zinc (53 % inhibition), 200 µM bupivacaine (55 % inhibition) and 1 mM quinidine (105 % inhibition).

5. The general anaesthetic halothane (1·5 %) increased the oxygen-sensitive K⁺ current (by 176 %). Halothane (3 mM) also stimulated single channel activity in inside-out patches (by 240 %). Chloroform had no effect on background K⁺ channel activity.

6. Acidosis (pH 6·4) inhibited the oxygen-sensitive background K⁺ current (by 56 %) and depolarised type-I cells.

7. The pharmacological and biophysical properties of the background K⁺ channel are, therefore, analogous to those of the cloned channel TASK-1. Using in situ hybridisation TASK-1 mRNA was found to be expressed in type-I cells. We conclude that the oxygen- and acid-sensitive background K⁺ channel of carotid body type-I cells is likely to be an endogenous TASK-1-like channel.

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