HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 538/1/53    most recent 2001.012794v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Abdrachmanova, G. Articles by Vyklicky, L. J. Search for Related Content PubMed PubMed Citation Articles by Abdrachmanova, G. Articles by Vyklicky, L. J.

Axotomy-induced changes in the properties of NMDA receptor channels in rat spinal cord motoneurons

Galya Abdrachmanova, Jan Teisinger and Ladislav Vyklicky Jr

Properties of N-methyl-D-aspartate (NMDA) receptor channels were studied using the patch-clamp technique in fluorescence-labelled control and axotomised motoneurons in thin spinal cord slices. Single-channel currents induced by NMDA in outside-out patches isolated from axotomised motoneurons and voltage clamped at -100 mV, exhibited six amplitude levels with a mean conductance of 14.9 ± 1.9, 22.2 ± 2.7, 35.6 ± 4.4, 49.1 ± 3.5, 59.6 ± 3.5 and 69.0 ± 2.9 pS. In contrast, the conductance of NMDA receptor channels, recorded under identical conditions in control motoneurons was characterised by only four levels corresponding to 20.1 ± 2.5, 38.0 ± 3.0, 58.6 ± 3.4 and 71.5 ± 2.6 pS. The time course of deactivation of NMDA receptor EPSCs in axotomised motoneurons voltage clamped at +40 mV was double exponential. The deactivation had a similar time course in control and axotomised motoneurons from 6-day-old animals; however, the deactivation became faster with increased time after injury. The fast and slow time constants in motoneurons 8 days after axotomy became three times faster than in controls. NMDA receptor-mediated responses were voltage dependent in the presence of extracellular Mg²⁺. In axotomised motoneurons Boltzmann analysis of the relationship between the peak amplitude of NMDA receptor EPSCs or NMDA-induced responses and membrane potential suggested an apparent K_d for Mg²⁺ binding (at 0 mV) of 1.2 ± 0.5 and 3.4 ± 3.9 mM, respectively. Single-cell RT-PCR analysis of mRNA revealed that NR2A-D and NR3A subunit transcripts were expressed in axotomised motoneurons. The results of our experiments suggest that in addition to genotypic control of NMDA receptors in motoneurons, axotomy, an experimental model of neurodegeneration, alters functional properties of the receptors in motoneurons destined to die.

This article has been cited by other articles:

				V. L. Arvanian, W. J. Bowers, J. C. Petruska, V. Motin, H. Manuzon, W. C. Narrow, H. J. Federoff, and L. M. Mendell Viral Delivery of NR2D Subunits Reduces Mg2+ Block of NMDA Receptor and Restores NT-3-Induced Potentiation of AMPA-Kainate Responses in Maturing Rat Motoneurons J Neurophysiol, October 1, 2004; 92(4): 2394 - 2404. [Abstract] [Full Text] [PDF]

				K. E. Hecker, J. H. Baumert, N. Horn, M. Reyle-Hahn, N. Heussen, and R. Rossaint Minimum Anesthetic Concentration of Sevoflurane with Different Xenon Concentrations in Swine Anesth. Analg., November 1, 2003; 97(5): 1364 - 1369. [Abstract] [Full Text] [PDF]

				K. E. Hecker, M. Reyle-Hahn, J. H. Baumert, N. Horn, N. Heussen, and R. Rossaint Minimum Alveolar Anesthetic Concentration of Isoflurane with Different Xenon Concentrations in Swine Anesth. Analg., January 1, 2003; 96(1): 119 - 124. [Abstract] [Full Text] [PDF]

				C. L. Jordan, S. E. Christensen, R. J. Handa, J. L. Anderson, W. A. Pouliot, and S. M. Breedlove Evidence That Androgen Acts Through NMDA Receptors to Affect Motoneurons in the Rat Spinal Nucleus of the Bulbocavernosus J. Neurosci., November 1, 2002; 22(21): 9567 - 9572. [Abstract] [Full Text] [PDF]